6LFT

Poa1p S30A mutant in complex with ADP-ribose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-10-14
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	P 64
Unit cell lengths	82.218, 82.218, 52.416
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.595 - 1.570
R-factor	0.1525
Rwork	0.151
R-free	0.16620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lfq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.595	1.620
High resolution limit [Å]	1.570	1.570
Number of reflections	27730
<I/σ(I)>	28.7
Completeness [%]	99.0
Redundancy	5.2
CC(1/2)	0.964	0.838

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283	Ammonium acetate, Sodium citrate tribasic dihydrate, PEG 4000, Glycerol