6LFR

Poa1p in complex with ADP-ribose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-26
Detector	RAYONIX MX300HE
Wavelength(s)	1
Spacegroup name	P 64
Unit cell lengths	85.619, 85.619, 37.945
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.518 - 1.780
R-factor	0.1781
Rwork	0.175
R-free	0.20290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lfq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.518	1.840
High resolution limit [Å]	1.780	1.780
Number of reflections	15302
<I/σ(I)>	51.3
Completeness [%]	99.2
Redundancy	9.7
CC(1/2)	0.985	0.917

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283	Ammonium sulfate, Sodium cacodylate trihydrate, PEG 8000, Glycerol