6LFJ

Crystal structure of mouse DCAR2 CRD domain complex with IPM2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2019-10-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	65.689, 72.564, 100.469
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.700 - 1.840
R-factor	0.1782
Rwork	0.177
R-free	0.19770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6kzr
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.700	1.940
High resolution limit [Å]	1.840	1.840
Number of reflections	80659	13015
<I/σ(I)>	5.6
Completeness [%]	99.6
Redundancy	3.5
CC(1/2)	0.990	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283	PEG