6LF1
SeviL, a GM1b/asialo-GM1 binding lectin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-25 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 86.216, 86.216, 67.627 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.110 - 1.700 |
R-factor | 0.1942 |
Rwork | 0.193 |
R-free | 0.22770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4i40 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.736 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | BALBES |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.110 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rpim | 0.038 | 0.385 |
Number of reflections | 371509 | 1641 |
<I/σ(I)> | 5.1 | |
Completeness [%] | 98.3 | 97.1 |
Redundancy | 11.7 | 12.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 293 | 0.1 M citric acid, pH 3.5, 3 M NaCl |