6LF0
Structure of FEM1C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-01-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 88.720, 98.480, 146.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.502 - 2.110 |
R-factor | 0.1966 |
Rwork | 0.195 |
R-free | 0.22910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6lbf |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.740 | 2.160 |
High resolution limit [Å] | 2.110 | 2.110 |
Number of reflections | 74445 | 7352 |
<I/σ(I)> | 19.7 | |
Completeness [%] | 99.9 | |
Redundancy | 13.4 | |
CC(1/2) | 0.999 | 0.820 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M Lithium sulfate, 0.1M Tris-HCl pH 7.3 |