6LEO
Crystal structure of thiosulfate transporter YeeE from Spirochaeta thermophila
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-15 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.44 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 73.510, 95.320, 101.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.134 - 2.520 |
R-factor | 0.2069 |
Rwork | 0.202 |
R-free | 0.25230 |
Structure solution method | SIRAS |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | AutoSol |
Refinement software | PHENIX (1.16) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.134 | 43.134 | 2.670 |
High resolution limit [Å] | 2.520 | 7.560 | 2.520 |
Rmerge | 0.631 | 0.097 | 6.752 |
Rmeas | 0.635 | 0.097 | 6.792 |
Total number of observations | 2039899 | ||
Number of reflections | 12366 | 866 | 3712 |
<I/σ(I)> | 14.76 | 63.15 | 1.89 |
Completeness [%] | 99.9 | 99.3 | 100 |
Redundancy | 87.621 | 99.119 | 85.562 |
CC(1/2) | 0.984 | 0.999 | 0.379 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 7 | 293 | Pentaerythritol-propoxylate, MES, NaCl |