6LEN

Structure of NS11 bound FEM1C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-07-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	95.727, 96.508, 146.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	80.652 - 2.383
R-factor	0.2006
Rwork	0.199
R-free	0.23460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lbf
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.652	2.510
High resolution limit [Å]	2.380	2.380
Number of reflections	54996	5405
<I/σ(I)>	16.8
Completeness [%]	100.0
Redundancy	13.1
CC(1/2)	0.998	0.823

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M Sodium citrate tribasic dihydrate, 0.1M Tris pH 8.2