6LED
Structure of D-carbamoylase mutant from Nitratireductor indicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2019-11-11 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 93.992, 93.992, 247.443 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.370 |
| R-factor | 0.1699 |
| Rwork | 0.168 |
| R-free | 0.21460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fo6 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.510 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.450 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Rmerge | 0.126 | 0.626 |
| Number of reflections | 27278 | 2647 |
| <I/σ(I)> | 37.17 | |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 20.8 | 18.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | PEG 4000, Sodium Phosphate, sodium chloride |
