6LEA

Structure of FliS chaperone in complex with flagellin and HP1076

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00
Spacegroup name	P 61
Unit cell lengths	103.330, 103.330, 144.211
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.620 - 2.950
R-factor	0.1954
Rwork	0.190
R-free	0.24730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3k1i
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.17rc1_3605)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.620	3.110
High resolution limit [Å]	2.950	2.950
Rmerge	0.091	0.452
Number of reflections	16741	2628
<I/σ(I)>	16.6
Completeness [%]	90.9
Redundancy	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	3% (v/v) PEG 20000, 0.2M Potassium bromide, 0.2M Potassium thiocyanate, 0.2M Sodium acetate pH 5.0