6LE4

Crystal structure of cystathionine gamma-lyase from Lactobacillus plantarum complexed with cystathionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-30
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	216.568, 200.738, 114.883
Unit cell angles	90.00, 117.21, 90.00

Refinement procedure

Resolution	36.257 - 3.100
R-factor	0.2471
Rwork	0.246
R-free	0.27250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdbid 4L0O
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	3.210
High resolution limit [Å]	3.083	6.680	3.100
Rmerge	0.142	0.061	0.434
Rmeas	0.189	0.081	0.575
Rpim	0.123	0.052	0.374
Number of reflections	72733	6935	7469
<I/σ(I)>	4.1
Completeness [%]	91.4	86.1	94.2
Redundancy	2	2.2	2
CC(1/2)		0.990	0.761

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	298	KH2PO4, NaH2PO4