6LE1
Structure of RRM2 domain of DND1 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.972 |
| Spacegroup name | I 41 |
| Unit cell lengths | 44.615, 44.615, 111.332 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.570 - 2.300 |
| R-factor | 0.2116 |
| Rwork | 0.210 |
| R-free | 0.24310 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.600 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.092 | 0.530 |
| Rmeas | 0.100 | |
| Rpim | 0.039 | |
| Number of reflections | 4233 | 237 |
| <I/σ(I)> | 15.7 | |
| Completeness [%] | 92.4 | 99.6 |
| Redundancy | 6.7 | 6.7 |
| CC(1/2) | 0.997 | 0.921 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M MES Monohydrate pH- 6.5, 30% PEG MME 5000, 0.2M Ammonium sulphate |
