6LDX
Structure antibody E6 in complex with methylated peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-16 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 82.329, 82.329, 120.269 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.940 - 1.800 |
| R-factor | 0.1713 |
| Rwork | 0.169 |
| R-free | 0.21730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ldv |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.857 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.940 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.092 | 0.468 |
| Rpim | 0.019 | 0.131 |
| Number of reflections | 39032 | 5534 |
| <I/σ(I)> | 23.3 | 5.6 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 23.2 | 13.5 |
| CC(1/2) | 0.999 | 0.875 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1 M potassium chloride 24% PEG3350 |
