6LDW
Structure of antibody C9 in complex with methylated peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-02-06 |
| Detector | RAYONIX MX225-HS |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.310, 75.680, 76.320 |
| Unit cell angles | 73.98, 87.16, 84.78 |
Refinement procedure
| Resolution | 39.160 - 1.600 |
| R-factor | 0.1732 |
| Rwork | 0.171 |
| R-free | 0.23480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ldv |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.580 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.700 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.040 | 0.346 |
| Rpim | 0.346 | |
| Number of reflections | 106948 | 15357 |
| <I/σ(I)> | 8.4 | 2.1 |
| Completeness [%] | 96.3 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2 M Magnesium chloride hexahydrate, 20% PEG3350 |
