6LDP

Structure of CDK5R1-bound FEM1C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-07-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	94.930, 96.620, 146.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.346 - 2.350
R-factor	0.2242
Rwork	0.222
R-free	0.25820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lbf
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.120	2.410
High resolution limit [Å]	2.350	2.350
Number of reflections	56771	5605
<I/σ(I)>	21.5
Completeness [%]	100.0
Redundancy	13.2
CC(1/2)	1.000	0.821

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M Lithium sulfate, 0.1M Tris-Hcl pH 7.9