6LDN
Crystal structure of T.onnurineus Csm5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2017-09-21 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 61.473, 131.072, 51.913 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.770 - 2.600 |
R-factor | 0.2134 |
Rwork | 0.208 |
R-free | 0.26330 |
Structure solution method | MIR |
RMSD bond length | 0.007 |
RMSD bond angle | 0.972 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.770 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 13324 | 13324 |
<I/σ(I)> | 61.49 | |
Completeness [%] | 99.6 | |
Redundancy | 13.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | 20 % PEG 8000 (w/v), 100 mM CHES/sodium hydroxide pH 9.5 |