6LD6
Structure of Bifidobacterium dentium beta-glucuronidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2013-07-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.583, 104.902, 160.620 |
| Unit cell angles | 90.00, 91.16, 90.00 |
Refinement procedure
| Resolution | 29.601 - 2.204 |
| R-factor | 0.1518 |
| Rwork | 0.151 |
| R-free | 0.20480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k46 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.788 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.290 |
| High resolution limit [Å] | 2.204 | 4.760 | 2.210 |
| Rmerge | 0.079 | 0.034 | 0.338 |
| Number of reflections | 154379 | 15864 | 13992 |
| <I/σ(I)> | 10.2 | ||
| Completeness [%] | 98.9 | 99.7 | 90.3 |
| Redundancy | 4.3 | 4.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M sodium cacodylate, pH 6.5, 8% w/v PEG 20K |
