6LCY
Crystal structure of Synaptotagmin-7 C2B in complex with IP6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 61 |
Unit cell lengths | 104.406, 104.406, 53.404 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.209 - 2.301 |
R-factor | 0.2034 |
Rwork | 0.202 |
R-free | 0.23870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3n5a |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP (3.25) |
Refinement software | PHENIX (v1.13) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 6.240 | 2.300 |
Rmerge | 0.079 | 0.035 | 0.518 |
Rmeas | 0.090 | 0.040 | 0.600 |
Rpim | 0.043 | 0.019 | 0.298 |
Total number of observations | 60777 | ||
Number of reflections | 14840 | 763 | 724 |
<I/σ(I)> | 10.3 | ||
Completeness [%] | 99.6 | 96.7 | 100 |
Redundancy | 4.1 | 3.8 | 3.9 |
CC(1/2) | 0.994 | 0.802 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 100mM NaCl, 50mM Tris-Cl, 1mM EDTA, 1mM DDT, 5mM IP6 |