6LCV
structure of Mutant S44P of maltooligosyltrehalose synthase from Arthrobacter ramosus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-21 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97930 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 194.683, 194.683, 200.354 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 21.240 - 2.840 |
R-factor | 0.1619 |
Rwork | 0.160 |
R-free | 0.18910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5zcr |
RMSD bond length | 0.008 |
RMSD bond angle | 0.971 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.890 |
High resolution limit [Å] | 2.840 | 2.840 |
Number of reflections | 53170 | 2582 |
<I/σ(I)> | 1.75 | |
Completeness [%] | 99.9 | |
Redundancy | 25.5 | |
CC(1/2) | 0.992 | 0.668 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | Ammonium citrate tribasic and BIS-TRIS propane |