6LCU

structure of maltooligosyltrehalose synthase from Arthrobacter ramosus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97919
Spacegroup name	P 61 2 2
Unit cell lengths	195.430, 195.430, 197.727
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.450
R-factor	0.1549
Rwork	0.154
R-free	0.17760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zcr
RMSD bond length	0.010
RMSD bond angle	1.039
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.450	2.450
Number of reflections	81790	4011
<I/σ(I)>	1.85
Completeness [%]	100.0
Redundancy	21
CC(1/2)	0.997	0.718

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	Succinic acid