6LCN

Crystal structure of Serine Acetyltransferase from Planctomyces limnophilus at 2.15A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-10-14
Detector	RIGAKU RAXIS
Wavelength(s)	1.5417
Spacegroup name	P 21 21 21
Unit cell lengths	84.719, 113.165, 230.133
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.596 - 2.150
Rwork	0.187
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3f1x
RMSD bond length	0.007
RMSD bond angle	1.475
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.596	2.227
High resolution limit [Å]	2.150	2.150
Rmerge	0.095	0.742
Rmeas	0.108	0.841
Rpim	0.050	0.391
Number of reflections	120745	11863
<I/σ(I)>	14.44
Completeness [%]	99.6	99.23
Redundancy	4.7	4.5
CC(1/2)	0.997	0.658

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	278	0.1M MES pH=6.5, 12% w/v PEG 20,000