6LCM
Crystal structure of chloroplast resolvase ZmMOC1 with the magic triangle I3C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-02 |
| Detector | DECTRIS PILATUS3 R 200K-A |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 49.136, 49.136, 141.831 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 2.500 |
| R-factor | 0.2256 |
| Rwork | 0.223 |
| R-free | 0.27990 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.684 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (v1.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.092 | 0.302 |
| Number of reflections | 6571 | 670 |
| <I/σ(I)> | 70.8 | |
| Completeness [%] | 100.0 | |
| Redundancy | 33.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | PEG 3350, Ammonium fluoride |
