6LCG
Structure of D-carbamoylase mutant from Nitratireductor indicus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 18-ID |
Synchrotron site | APS |
Beamline | 18-ID |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2019-11-11 |
Detector | Bruker AXIOM 200 |
Wavelength(s) | 0.987 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 95.650, 95.650, 261.621 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.820 - 2.700 |
R-factor | 0.2184 |
Rwork | 0.216 |
R-free | 0.26550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fo6 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.426 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.820 | 2.830 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.229 | 2.257 |
Number of reflections | 20291 | 2616 |
<I/σ(I)> | 1.51 | |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 21 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | PEG 4000, Sodium Chlorium, Tris-HCl |