6LCF
Crystal Structure of beta-L-arabinobiose binding protein - native
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.353, 52.957, 102.766 |
| Unit cell angles | 90.00, 99.70, 90.00 |
Refinement procedure
| Resolution | 46.970 - 1.920 |
| R-factor | 0.1834 |
| Rwork | 0.182 |
| R-free | 0.21440 |
| Structure solution method | SAD |
| RMSD bond length | 0.032 |
| RMSD bond angle | 1.684 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.970 | 1.970 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.116 | 0.540 |
| Number of reflections | 56566 | 3817 |
| <I/σ(I)> | 6 | 1.9 |
| Completeness [%] | 100.0 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.993 | 0.789 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 293 | 18% PEG 1000, 0.1M Na-citrate (pH 3.5), 0.6mM beta-L-arabinobiose |
