6LCE
Crystal Structure of beta-L-arabinobiose binding protein - selenomethionine derivative
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-13 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9789 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.339, 66.277, 92.118 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.200 - 1.780 |
R-factor | 0.1415 |
Rwork | 0.139 |
R-free | 0.18220 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.749 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.200 | 1.820 |
High resolution limit [Å] | 1.780 | 1.780 |
Rmerge | 0.161 | 0.947 |
Number of reflections | 31327 | 1476 |
<I/σ(I)> | 18.3 | 2.3 |
Completeness [%] | 98.0 | |
Redundancy | 17.4 | |
CC(1/2) | 0.998 | 0.759 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 293 | 18% PEG 1000, 0.1M Na-citrate (pH 3.5), 0.6mM beta-L-arabinobiose |