6LBS

Crystal structure of yeast Stn1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	C 1 2 1
Unit cell lengths	158.425, 94.749, 97.498
Unit cell angles	90.00, 96.26, 90.00

Refinement procedure

Resolution	37.946 - 2.600
R-factor	0.1857
Rwork	0.183
R-free	0.23490
Structure solution method	SAD
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.690
High resolution limit [Å]	2.600	5.600	2.600
Rmerge	0.069	0.031	0.722
Rmeas	0.075	0.033	0.783
Rpim	0.029	0.013	0.300
Number of reflections	84248	4572	4446
<I/σ(I)>	6.8
Completeness [%]	99.9	99.9	100
Redundancy	6.8	6.9	6.8
CC(1/2)		0.997	0.808

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4	277	0.1 M citric acid, 0.8 M ammonium sulfate