6LBR

Crystal structure of yeast Cdc13 and ssDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	75.166, 106.955, 173.521
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.840 - 2.500
R-factor	0.2041
Rwork	0.202
R-free	0.25180
Structure solution method	SAD
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.590
High resolution limit [Å]	2.500	5.380	2.500
Rmerge	0.104	0.075	0.843
Rmeas	0.115	0.084	0.927
Rpim	0.050	0.038	0.379
Total number of observations	270829
Number of reflections	49182	5190	4843
<I/σ(I)>	5.9
Completeness [%]	99.8	99.7	99.8
Redundancy	5.5	5.3	5.7
CC(1/2)		0.990	0.769

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		277	25% Ethylene Glycol, 0.01 mM EDTA and 150 mM NaCl