6LBP

Structure of the Glutamine Phosphoribosylpyrophosphate Amidotransferase from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-01-05
Detector	MAR CCD 130 mm
Wavelength(s)	0.979
Spacegroup name	P 31 2 1
Unit cell lengths	179.736, 179.736, 109.199
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.656 - 3.065
R-factor	0.1911
Rwork	0.189
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gph
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.656	3.175
High resolution limit [Å]	3.065	3.065
Rmerge	0.099	0.732
Number of reflections	38107	3526
<I/σ(I)>	18.25	3.11
Completeness [%]	99.0
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	0.1 M Sodium citrate tribasic pH 5.6, 1.0 M Ammonium phosphate monobasic