6LBN

Structure of SIL1-bound FEM1C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-05-13
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	94.288, 97.379, 146.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.039 - 2.899
R-factor	0.2241
Rwork	0.222
R-free	0.25810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lbf
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.899	2.899
Number of reflections	30698	1961
<I/σ(I)>	22
Completeness [%]	99.8
Redundancy	13.1
CC(1/2)	0.999	0.688

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.3M Lithium sulfate, 0.1M Tris-HCL pH 8.5, 5% v/v Polyethylene glycol 400