6LBJ

Structure of mouse GLD-2 (Terminal nucleotidyltransferase 2, TENT2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-09
Detector	RAYONIX MX-225
Wavelength(s)	0.918003
Spacegroup name	P 1
Unit cell lengths	42.277, 47.144, 105.446
Unit cell angles	87.38, 88.06, 63.81

Refinement procedure

Resolution	35.514 - 2.704
R-factor	0.253956762327
Rwork	0.253
R-free	0.27084
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jnb
RMSD bond length	0.008
RMSD bond angle	1.162
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MrBUMP
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.280	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.091
Number of reflections	17614	1695
<I/σ(I)>	11.2
Completeness [%]	88.3	83.5
Redundancy	2.6
CC(1/2)	0.977	0.835

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	291.15	23.6% PEG 3350, 80mM HEPEs pH 7.4