6LBG

Structure of OR51B2 bound FEM1C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-10-18
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	87.918, 97.690, 146.197
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.555 - 2.510
R-factor	0.1977
Rwork	0.196
R-free	0.23110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lbf
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	87.890	2.580
High resolution limit [Å]	2.510	2.510
Number of reflections	43610	4267
<I/σ(I)>	9.4
Completeness [%]	100.0
Redundancy	12.9
CC(1/2)	0.998	0.886

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M Tris-HCl, pH 7.0, 0.8M Lithium sulfate