6LB9
Magnesium ion-bound SspB crystal structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 193.15 |
Detector technology | PIXEL |
Collection date | 2015-07-07 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.978 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.248, 80.249, 146.022 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.605 - 2.227 |
R-factor | 0.1987 |
Rwork | 0.196 |
R-free | 0.25240 |
Structure solution method | SAD |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.010 | 2.300 |
High resolution limit [Å] | 2.220 | 2.220 |
Number of reflections | 33730 | 30780 |
<I/σ(I)> | 8.5 | |
Completeness [%] | 99.7 | |
Redundancy | 6.3 | |
CC(1/2) | 0.992 | 0.993 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 287.15 | 25% PEG 3350,0.2M Magnesium chloride |