6LAN
Structure of CCDC50 and LC3B complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97930 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.776, 46.804, 46.154 |
| Unit cell angles | 90.00, 101.27, 90.00 |
Refinement procedure
| Resolution | 23.400 - 1.410 |
| R-factor | 0.1938 |
| Rwork | 0.192 |
| R-free | 0.21890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wao |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.801 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.430 |
| High resolution limit [Å] | 1.410 | 1.410 |
| Number of reflections | 24956 | 1672 |
| <I/σ(I)> | 49.9 | 1.8 |
| Completeness [%] | 97.5 | 91.3 |
| Redundancy | 12 | 8.7 |
| CC(1/2) | 0.997 | 0.902 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 294 | 20% v/v 2-Propanol, 0.1 M sodium citrate pH 5.6, 20% w/v PEG 2000 MME |
