6LAF
Crystal structure of the core domain of Amuc_1100 from Akkermansia muciniphila
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-19 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 2 2 21 |
Unit cell lengths | 59.746, 85.538, 86.413 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.603 - 3.001 |
R-factor | 0.2588 |
Rwork | 0.257 |
R-free | 0.29780 |
Structure solution method | SAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
Rmerge | 0.268 | 0.062 | 1.458 |
Rmeas | 0.283 | 0.066 | 1.528 |
Rpim | 0.088 | 0.021 | 0.453 |
Total number of observations | 96539 | ||
Number of reflections | 9314 | 1024 | 892 |
<I/σ(I)> | 3.2 | ||
Completeness [%] | 99.9 | 99.7 | 100 |
Redundancy | 10.4 | 9.5 | 11.3 |
CC(1/2) | 0.998 | 0.849 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 2 M AmSO4 |