6LAF

Crystal structure of the core domain of Amuc_1100 from Akkermansia muciniphila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-19
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9793
Spacegroup name	P 2 2 21
Unit cell lengths	59.746, 85.538, 86.413
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.603 - 3.001
R-factor	0.2588
Rwork	0.257
R-free	0.29780
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.110
High resolution limit [Å]	3.000	6.460	3.000
Rmerge	0.268	0.062	1.458
Rmeas	0.283	0.066	1.528
Rpim	0.088	0.021	0.453
Total number of observations	96539
Number of reflections	9314	1024	892
<I/σ(I)>	3.2
Completeness [%]	99.9	99.7	100
Redundancy	10.4	9.5	11.3
CC(1/2)		0.998	0.849

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	2 M AmSO4