6LAB

169 bp nucleosome, harboring cohesive DNA termini, assembled with linker histone H1.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	98.15
Detector technology	PIXEL
Collection date	2015-09-18
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	104.799, 102.760, 218.050
Unit cell angles	90.00, 97.40, 90.00

Refinement procedure

Resolution	39.892 - 3.200
Rwork	0.201
R-free	0.26190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ut9 4qlc
RMSD bond length	0.006
RMSD bond angle	1.289
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	216.230	3.370
High resolution limit [Å]	3.200	3.200
Rmerge	0.108	0.811
Rmeas	0.151
Rpim	0.105
Number of reflections	75580	10654
<I/σ(I)>	5.3	1.2
Completeness [%]	99.3	96.6
Redundancy	3	2.8
CC(1/2)	0.993	0.557

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	291.15	Calcium chloride, potassium chloride, sodium acetate