6LA0
Crystal structure of AoRut
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-22 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.908, 169.989, 50.485 |
Unit cell angles | 90.00, 115.62, 90.00 |
Refinement procedure
Resolution | 19.570 - 1.750 |
R-factor | 0.1975 |
Rwork | 0.196 |
R-free | 0.23020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2pf0 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.815 |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.152) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.850 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.097 | 0.747 |
Number of reflections | 72840 | 11345 |
<I/σ(I)> | 13.28 | 2.06 |
Completeness [%] | 98.9 | 95.4 |
Redundancy | 5.8 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 12% PEG4000, 0.5 M NaAcetate pH4.6 |