6L97
Complex of DNA polymerase IV and L-DNA duplex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9794 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 85.861, 54.969, 96.545 |
Unit cell angles | 90.00, 113.39, 90.00 |
Refinement procedure
Resolution | 34.496 - 2.362 |
R-factor | 0.232 |
Rwork | 0.230 |
R-free | 0.26580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bq3 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15_3459) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.440 |
High resolution limit [Å] | 2.360 | 5.080 | 2.360 |
Rmerge | 0.125 | 0.080 | 0.555 |
Rmeas | 0.140 | 0.090 | 0.656 |
Rpim | 0.062 | 0.041 | 0.342 |
Total number of observations | 153932 | ||
Number of reflections | 33537 | 3352 | 3238 |
<I/σ(I)> | 18.4 | ||
Completeness [%] | 97.9 | 94.6 | 95.3 |
Redundancy | 4.6 | 5 | 3 |
CC(1/2) | 0.989 | 0.164 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.1 M Sodium formate, 0.05 M BICINE (pH 8.5), 10% w/v Polyethylene glycol monomethyl ether 5,000, and 1.1% benzamidine-HCl |