6L93

X-ray structure of the ligand-free human TRPV1 ankyrin repeat domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2019-06-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	184.228, 167.414, 112.632
Unit cell angles	90.00, 108.38, 90.00

Refinement procedure

Resolution	32.950 - 4.470
R-factor	0.2227
Rwork	0.220
R-free	0.26980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pnn
RMSD bond length	0.002
RMSD bond angle	0.480
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.950	4.630
High resolution limit [Å]	4.470	4.470
Number of reflections	19438	4678
<I/σ(I)>	6.4
Completeness [%]	98.7
Redundancy	3.4
CC(1/2)	0.993	0.817

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277	0.125 M sodium citrate (pH 5.0), 5% PEG 8000, 2.5% (v/v) glycerol