6L8S
High resolution crystal structure of crustacean hemocyanin.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-11-30 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 119.454, 207.596, 187.364 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.695 - 1.580 |
R-factor | 0.1507 |
Rwork | 0.149 |
R-free | 0.18620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hcy |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.695 | 1.680 |
High resolution limit [Å] | 1.580 | 1.580 |
Rmerge | 0.464 | |
Number of reflections | 615236 | 99005 |
<I/σ(I)> | 15.24 | |
Completeness [%] | 99.7 | |
Redundancy | 3.78 | |
CC(1/2) | 0.754 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | Lithium sulfate monohydrate, Sodium citrate tribasic dihydrate, PEG1,000, magnesium chloride, |