6L8K
Structure of URT1 in complex with UTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.766, 64.928, 66.371 |
| Unit cell angles | 90.00, 121.19, 90.00 |
Refinement procedure
| Resolution | 30.627 - 2.999 |
| R-factor | 0.1927 |
| Rwork | 0.190 |
| R-free | 0.23960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5w0n |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.540 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.627 | 3.400 |
| High resolution limit [Å] | 2.999 | 2.999 |
| Rmerge | 0.106 | 0.499 |
| Number of reflections | 8925 | 2924 |
| <I/σ(I)> | 8.857 | |
| Completeness [%] | 98.6 | |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M HEPES PH7.5, 10% PEG6000, 5% MPD |
