6L8G

High resolution structure of YoeB in complex with YefM C-terminus(46N-83V) from Staphylococcus aureus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	31.206, 58.117, 65.597
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.500 - 1.000
R-factor	0.1683
Rwork	0.168
R-free	0.17580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.754
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.020
High resolution limit [Å]	1.000	1.000
Rmerge	0.038	0.175
Rmeas	0.040	0.187
Rpim	0.011	0.063
Number of reflections	64910	3023
<I/σ(I)>	61.6	12.9
Completeness [%]	99.3
Redundancy	11.8
CC(1/2)	1.000	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	5% v/v Tacsimate pH 7.0 0.1 M HEPES pH 7.0 10% w/v Polyethylene glycol monomethyl ether 5,000