6L8F

Crystal structure of heterotetrameric complex of YoeB-YefM toxin-antitoxin from Staphylococcus aureus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97791
Spacegroup name	P 61
Unit cell lengths	104.438, 104.438, 172.586
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	62.430 - 2.400
R-factor	0.1912
Rwork	0.190
R-free	0.22340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2a6q
RMSD bond length	0.006
RMSD bond angle	0.835
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.174	1.439
Rmeas	0.179	1.480
Rpim	0.039	0.341
Number of reflections	41475	4340
<I/σ(I)>	15.5	2.5
Completeness [%]	99.9
Redundancy	20.5
CC(1/2)	0.999	0.827

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.07 M Citric acid, 0.03 M BIS-TRIS propane, pH 3.4, 16% w/v Polyethylene glycol 3350