6L8F
Crystal structure of heterotetrameric complex of YoeB-YefM toxin-antitoxin from Staphylococcus aureus.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-20 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97791 |
Spacegroup name | P 61 |
Unit cell lengths | 104.438, 104.438, 172.586 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 62.430 - 2.400 |
R-factor | 0.1912 |
Rwork | 0.190 |
R-free | 0.22340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2a6q |
RMSD bond length | 0.006 |
RMSD bond angle | 0.835 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX (1.18.2_3874) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.174 | 1.439 |
Rmeas | 0.179 | 1.480 |
Rpim | 0.039 | 0.341 |
Number of reflections | 41475 | 4340 |
<I/σ(I)> | 15.5 | 2.5 |
Completeness [%] | 99.9 | |
Redundancy | 20.5 | |
CC(1/2) | 0.999 | 0.827 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.07 M Citric acid, 0.03 M BIS-TRIS propane, pH 3.4, 16% w/v Polyethylene glycol 3350 |