6L8E

Crystal structure of heterohexameric YoeB-YefM complex bound to 26bp-DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97918
Spacegroup name	P 43 21 2
Unit cell lengths	151.352, 151.352, 183.778
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.510 - 2.350
R-factor	0.1985
Rwork	0.197
R-free	0.22250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2a6q
RMSD bond length	0.004
RMSD bond angle	0.658
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.480	2.390
High resolution limit [Å]	2.350	2.350
Rmerge	0.078	1.070
Rmeas	0.083	1.147
Rpim	0.030	0.403
Number of reflections	89409	4434
<I/σ(I)>	17.4	2.5
Completeness [%]	99.8
Redundancy	7.4
CC(1/2)	0.999	0.791

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	0.1 M HEPES pH 7.5, 10% w/v Polyethylene glycol 8000, 8% v/v Ethylene glycol