6L8D
Hexameric structure of the ATPase subunit of magnesium chelatase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 211.034, 121.149, 119.419 |
Unit cell angles | 90.00, 99.06, 90.00 |
Refinement procedure
Resolution | 49.330 - 2.910 |
R-factor | 0.234 |
Rwork | 0.233 |
R-free | 0.25200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g8p |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.076 | 0.731 |
Number of reflections | 64295 | 6437 |
<I/σ(I)> | 17.8 | 2 |
Completeness [%] | 98.5 | |
Redundancy | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | sodium malonate, PEG 3,350. |