6L85
The sodium-dependent phosphate transporter
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-04-03 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 0.9998 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 121.178, 112.650, 110.792 |
Unit cell angles | 90.00, 119.36, 90.00 |
Refinement procedure
Resolution | 29.399 - 2.302 |
R-factor | 0.1898 |
Rwork | 0.188 |
R-free | 0.22410 |
Structure solution method | SAD |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.380 |
High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
Rmerge | 0.044 | 0.024 | 0.355 |
Rmeas | 0.052 | 0.028 | 0.423 |
Rpim | 0.026 | 0.014 | 0.227 |
Total number of observations | 208830 | ||
Number of reflections | 56236 | 5742 | 5193 |
<I/σ(I)> | 12.4 | ||
Completeness [%] | 98.1 | 98.2 | 91.2 |
Redundancy | 3.7 | 3.7 | 3 |
CC(1/2) | 0.999 | 0.875 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 50mM sodium citrate (pH 5.5), 100mM NaCl, 18-20% (w/v) pentaerythritol propoxylate (5/4 PO/OH) and 10-12% polyethylene glycol 300 |