6L82
Crystal structure of Chaetomium GCP5 N-terminus and Mozart1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-11-16 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 0.9998 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 83.760, 101.878, 51.670 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.903 - 2.241 |
R-factor | 0.21587415806 |
Rwork | 0.211 |
R-free | 0.26309 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6l7r |
RMSD bond length | 0.010 |
RMSD bond angle | 0.973 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.330 |
High resolution limit [Å] | 2.200 | 2.250 |
Number of reflections | 77073 | 10652 |
<I/σ(I)> | 14.2 | |
Completeness [%] | 97.3 | |
Redundancy | 7.2 | |
CC(1/2) | 0.997 | 0.923 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1% tryptone, 0.05 M HEPES sodium pH 7.0, 0.001 M sodium azide, 20% PEG 3350 |