6L80
Crystal structure of pombe Mod21 N-terminus and Mozart1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-04-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9794 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 83.513, 83.513, 122.792 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.526 - 2.000 |
R-factor | 0.201863724302 |
Rwork | 0.200 |
R-free | 0.23460 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.763 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 267023 | 29883 |
<I/σ(I)> | 23.1 | |
Completeness [%] | 99.8 | |
Redundancy | 8.9 | |
CC(1/2) | 0.995 | 0.927 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 60% v/v Tacsimate pH 7.0, 0.1 M Bis-Tris propane pH 7.0 |