6L7T
Crystal structure of FKRP in complex with Mg ion, Zinc low remote data
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-06 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.2898 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.999, 119.480, 257.789 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.050 - 2.410 |
R-factor | 0.1793 |
Rwork | 0.178 |
R-free | 0.21040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6kan |
RMSD bond length | 0.006 |
RMSD bond angle | 0.832 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.050 | 2.450 |
High resolution limit [Å] | 2.410 | 2.410 |
Rmerge | 0.090 | 0.727 |
Rmeas | 0.098 | 0.892 |
Number of reflections | 177638 | 4368 |
<I/σ(I)> | 16.2 | 2.2 |
Completeness [%] | 99.8 | |
Redundancy | 6.6 | |
CC(1/2) | 0.999 | 0.769 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 4-8% PEG 4000, 0.1 M HEPES-NaOH (pH 7.5), 0.2 M MgCl2 |