6L7T

Crystal structure of FKRP in complex with Mg ion, Zinc low remote data

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-06
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.2898
Spacegroup name	P 21 21 21
Unit cell lengths	76.999, 119.480, 257.789
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.050 - 2.410
R-factor	0.1793
Rwork	0.178
R-free	0.21040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6kan
RMSD bond length	0.006
RMSD bond angle	0.832
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.050	2.450
High resolution limit [Å]	2.410	2.410
Rmerge	0.090	0.727
Rmeas	0.098	0.892
Number of reflections	177638	4368
<I/σ(I)>	16.2	2.2
Completeness [%]	99.8
Redundancy	6.6
CC(1/2)	0.999	0.769

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	4-8% PEG 4000, 0.1 M HEPES-NaOH (pH 7.5), 0.2 M MgCl2