6L7R
Crystal structure of Chaetomium GCP3 N-terminus and Mozart1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-03-06 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 0.9784 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 69.515, 61.989, 52.244 |
| Unit cell angles | 90.00, 119.20, 90.00 |
Refinement procedure
| Resolution | 19.952 - 1.848 |
| R-factor | 0.191834798649 |
| Rwork | 0.188 |
| R-free | 0.22582 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.793 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.920 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Number of reflections | 101437 | 1621 |
| <I/σ(I)> | 26.1 | |
| Completeness [%] | 99.5 | |
| Redundancy | 6 | |
| CC(1/2) | 0.995 | 0.973 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2 M lithium sulfate monohydrate, 0.1 M Tris hydrochloride pH 8.5, 30% PEG 4000 |
