6L7H
crystal structure of GgCGT in complex with UDP and Nothofagin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-31 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.54056 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.193, 71.306, 110.022 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 9.002 - 1.800 |
R-factor | 0.1717 |
Rwork | 0.169 |
R-free | 0.21930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6l5p |
Data reduction software | CrysalisPro |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 59.838 | 9.002 | 1.900 |
High resolution limit [Å] | 1.800 | 5.690 | 1.800 |
Rmerge | 0.057 | 0.621 | |
Rmeas | 0.107 | 0.061 | 0.703 |
Rpim | 0.041 | 0.022 | 0.322 |
Total number of observations | 256181 | 7794 | 26316 |
Number of reflections | 39867 | 1029 | 5750 |
<I/σ(I)> | 12.5 | 28.5 | 2.1 |
Completeness [%] | 99.0 | 74.4 | 99.3 |
Redundancy | 6.4 | 7.6 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 283 | 0.2 M Ammonium acetate, 0.1 M BIS-Tris, pH 6.5, 25% w/v Polyethylene glycol 3350 |