6L77
Crystal structure of MS5 from Brassica napus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 52.672, 52.672, 168.424 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.620 - 1.900 |
| R-factor | 0.1761 |
| Rwork | 0.174 |
| R-free | 0.21940 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.949 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.620 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.120 | 0.472 |
| Rmeas | 0.122 | 0.482 |
| Rpim | 0.020 | 0.095 |
| Number of reflections | 21999 | 1217 |
| <I/σ(I)> | 27.3 | 7.7 |
| Completeness [%] | 98.9 | 97.2 |
| Redundancy | 36 | 24 |
| CC(1/2) | 0.999 | 0.972 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | Potassium sodium tartrate tetrahydrate, PEG 3350 |
